polyclonal antibody against opa1  (Proteintech)

Journal: Molecular Neurobiology

Article Title: Mesenchymal Stem Cell-derived Exosomes Alleviate Oxidative Stress and Brain Injuries Through Promoting OPA1 Mediated Mitochondrial Fusion After Intracerebral Hemorrhage

doi: 10.1007/s12035-026-05703-4

Figure Lengend Snippet: MSC-Exo treatment restored normalized mitochondrial dynamics by promoting OPA1 expression after ICH in vivo. A Representative images of mitochondrial morphology were captured using transmission electron microscopy, with scale bars:2 µm. B The mitochondria were categorized based on their length into three groups: <0.6 µm, 0.6–1.0 µm, and >1 µm ( n = 6 per group, one-way ANOVA). C Percentage of damaged mitochondria was calculated ( n = 6 per group, one-way ANOVA). D Representative western blots and quantitative results of OPA1, DRP1, and Mfn1/Mfn2 in the brain of mice. E–H The corresponding western blot data were statistically analyzed, and the results are displayed on the right side of the figures ( n = 6 per group, one-way ANOVA). Data are presented as mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001

Article Snippet: After being immersed in 5% non-fat milk for 2 h, the membranes were treated with primary rabbit polyclonal antibodies against OPA1 (#80,471, CST, Danvers, MA, USA), DRP1 (#8570, CST, Danvers, MA, USA), Mitofusin-1 (13,798–1-AP, Proteintech, Rosemont, Illinois, USA), Mitofusin-2 (12,186–1-AP, Proteintech, Rosemont, Illinois, USA), CD81 (27,855–1-AP, Proteintech, Rosemont, Illinois, USA), TSG101 (28,283–1-AP, Proteintech, Rosemont, Illinois, USA), GM130 (PA5-95,727, Invitrogen, Carlsbad, CA, USA) overnight at 4 °C.

Techniques: Expressing, In Vivo, Transmission Assay, Electron Microscopy, Western Blot

Journal: Molecular Neurobiology

Article Title: Mesenchymal Stem Cell-derived Exosomes Alleviate Oxidative Stress and Brain Injuries Through Promoting OPA1 Mediated Mitochondrial Fusion After Intracerebral Hemorrhage

doi: 10.1007/s12035-026-05703-4

Figure Lengend Snippet: Knockdown of Opa1 with siRNA blunted the protective effects of MSC-Exo in vitro. A Representative confocal microscopy images demonstrating mitochondrial morphology stained with MitoTracker are presented. B Relative aspect ratio statistical analysis was calculated ( n = 6 biological replicates, one-way ANOVA). C, D Representative images and quantitative appraisal of MitoSOX-stained mitochondria-associated superoxide generation are detailed ( n = 6 biological replicates, one-way ANOVA). E, F Effects of MSC-Exo treatment on MMP levels and NAD+/NADH levels ( n = 6 biological replicates, one-way ANOVA). Data are presented as mean ± SD, ** p < 0.01, *** p < 0.001, and **** p < 0.0001, ns, no statistical significance

Article Snippet: After being immersed in 5% non-fat milk for 2 h, the membranes were treated with primary rabbit polyclonal antibodies against OPA1 (#80,471, CST, Danvers, MA, USA), DRP1 (#8570, CST, Danvers, MA, USA), Mitofusin-1 (13,798–1-AP, Proteintech, Rosemont, Illinois, USA), Mitofusin-2 (12,186–1-AP, Proteintech, Rosemont, Illinois, USA), CD81 (27,855–1-AP, Proteintech, Rosemont, Illinois, USA), TSG101 (28,283–1-AP, Proteintech, Rosemont, Illinois, USA), GM130 (PA5-95,727, Invitrogen, Carlsbad, CA, USA) overnight at 4 °C.

Techniques: Knockdown, In Vitro, Confocal Microscopy, Staining

Journal: Molecular Neurobiology

Article Title: Mesenchymal Stem Cell-derived Exosomes Alleviate Oxidative Stress and Brain Injuries Through Promoting OPA1 Mediated Mitochondrial Fusion After Intracerebral Hemorrhage

doi: 10.1007/s12035-026-05703-4

Figure Lengend Snippet: Neuron-specific Opa1 deletion blunted MSC-Exo protection of mitochondrial dynamics in vivo. A Representative images showcasing mitochondrial morphology as captured by transmission electron microscopy, scale bars:1 µm. B Mitochondrial fusion was assessed by Mitochondria length (<0.6 µm, 0.6–1.0 µm, >1 µm) ( n = 6 per group, two-way ANOVA). C Percentage of damaged mitochondria was calculated ( n = 6 per group, two-way ANOVA). D–G Effects of Opa1 knockout and MSC-Exo treatment on ATP levels, NAD+/NADH levels, and normalized complex I/II activity in the hippocampus ( n = 6 per group, two-way ANOVA). Data are presented as mean ± SD, ** p < 0.01 and *** p < 0.001, ns, no statistical significance

Article Snippet: After being immersed in 5% non-fat milk for 2 h, the membranes were treated with primary rabbit polyclonal antibodies against OPA1 (#80,471, CST, Danvers, MA, USA), DRP1 (#8570, CST, Danvers, MA, USA), Mitofusin-1 (13,798–1-AP, Proteintech, Rosemont, Illinois, USA), Mitofusin-2 (12,186–1-AP, Proteintech, Rosemont, Illinois, USA), CD81 (27,855–1-AP, Proteintech, Rosemont, Illinois, USA), TSG101 (28,283–1-AP, Proteintech, Rosemont, Illinois, USA), GM130 (PA5-95,727, Invitrogen, Carlsbad, CA, USA) overnight at 4 °C.

Techniques: In Vivo, Transmission Assay, Electron Microscopy, Knock-Out, Activity Assay

Journal: Molecular Neurobiology

Article Title: Mesenchymal Stem Cell-derived Exosomes Alleviate Oxidative Stress and Brain Injuries Through Promoting OPA1 Mediated Mitochondrial Fusion After Intracerebral Hemorrhage

doi: 10.1007/s12035-026-05703-4

Figure Lengend Snippet: Neuron-specific Opa1 deletion blunted the protective effects of MSC-Exo treatment on oxidative stress and neurological outcomes after ICH. A, B Representative images depicting DHE staining and the quantitative assessment of DHE fluorescence intensity within the perilesional domain at 24 h post-intracerebral hemorrhage (ICH) are showcased, with a scale bar of 100 µm ( n = 6 per group, two-way ANOVA). C Effects of MSC-Exo treatment on MDA levels ( n = 6 per group, two-way ANOVA). D, F The reversal of the neuroprotective effects conferred by MSC-Exo in the context of neuronal-specific Opa1 conditional knockout is evaluated through behavioral assessments at various time points post-ICH, encompassing the Grid-walking test, Adhesive removal test, and Cylinder test ( n = 12 per group, two-way ANOVA). G The spatial learning and memory of subjects is further evaluated using the Morris water maze, portraying characteristic swim trajectories during the learning and memory phases of the assessment. H Mean swimming speed of mice in the MWM ( n = 12 per group, two-way ANOVA). I Spatial learning was evaluated through the measurement of escape latency in the Morris water maze from days 21 to 25 post-ICH ( n = 12 per group, two-way ANOVA). J, K Spatial memory is gauged by the duration spent in the target quadrant and the number of platform crossovers on day 26 following ICH ( n = 12 per group, two-way ANOVA). Data are presented as mean ± SD, ** p < 0.01, *** p < 0.001 and **** p < 0.0001

Article Snippet: After being immersed in 5% non-fat milk for 2 h, the membranes were treated with primary rabbit polyclonal antibodies against OPA1 (#80,471, CST, Danvers, MA, USA), DRP1 (#8570, CST, Danvers, MA, USA), Mitofusin-1 (13,798–1-AP, Proteintech, Rosemont, Illinois, USA), Mitofusin-2 (12,186–1-AP, Proteintech, Rosemont, Illinois, USA), CD81 (27,855–1-AP, Proteintech, Rosemont, Illinois, USA), TSG101 (28,283–1-AP, Proteintech, Rosemont, Illinois, USA), GM130 (PA5-95,727, Invitrogen, Carlsbad, CA, USA) overnight at 4 °C.

Techniques: Staining, Fluorescence, Knock-Out, Adhesive

Journal: International journal of molecular sciences

Article Title: Chrono-Aerobic Exercise Optimizes Metabolic State in DB/DB Mice through CLOCK-Mitophagy-Apoptosis.

doi: 10.3390/ijms23169308

Figure Lengend Snippet: Figure 5. Diabetes dramatically impairs mitochondrial quality, and exercise ameliorates excessive mitophagy caused by diabetes. (A) WesTM of OPA1, Mfn1, Drp1, and Fis1 expression in livers from

Article Snippet: Polyclonal antibodies against OPA1 (sc-367890, Santa Cruz, 1:50), Mfn1 (ab57602, Abcam, 1:250), Drp1 (ab184248, Abcam, 1:250), Fis1 (10956-1-AP, Proteintech, 1:85), HSP90 (ab178854, Abcam, 1:250), β-Tubulin (AF7011, Proteintech, 1:50), and horseradish peroxidase-conjugated secondary antibodies were diluted 1:25 (Santa Cruz Biotechnology Inc., Dallas, TX, USA).

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: Chrono-Aerobic Exercise Optimizes Metabolic State in DB/DB Mice through CLOCK-Mitophagy-Apoptosis.

doi: 10.3390/ijms23169308

Figure Lengend Snippet: Figure 6. The CLOCK expression is negatively correlated with Fis1 and OPA1 expression. ** p < 0.01 and *** p < 0.001.

Article Snippet: Polyclonal antibodies against OPA1 (sc-367890, Santa Cruz, 1:50), Mfn1 (ab57602, Abcam, 1:250), Drp1 (ab184248, Abcam, 1:250), Fis1 (10956-1-AP, Proteintech, 1:85), HSP90 (ab178854, Abcam, 1:250), β-Tubulin (AF7011, Proteintech, 1:50), and horseradish peroxidase-conjugated secondary antibodies were diluted 1:25 (Santa Cruz Biotechnology Inc., Dallas, TX, USA).

Techniques: Expressing